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Product Name
Recombinant MCL1 protein (His tag)
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Description
Recombinant human Mcl-1 protein fragment (residues 169-327) offers a stable and soluble unit ideal for structural biology and biochemical studies. This fragment, derived from the apoptosis regulator Induced myeloid leukemia cell differentiation protein Mcl-1, is designed to support research on cell survival mechanisms without the complexities of the full-length protein.
Mcl-1 (UniProt ID: Q07820) is a key regulator of apoptosis and cell survival in humans, functioning through interactions with other apoptotic proteins. While isoform 1 inhibits apoptosis, isoform 2 promotes it, highlighting its dual role in cellular viability. The full-length protein localizes to the membrane (as a single-pass protein), cytoplasm, mitochondria, and nucleoplasm, indicating its broad involvement in cellular processes. This recombinant fragment provides a focused tool for investigating Mcl-1's mechanisms in a soluble format, aiding studies on cancer and cell death pathways.
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Protein name
Induced myeloid leukemia cell differentiation protein Mcl-1
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Uniprot ID
Q07820
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Gene Name
MCL1; BCL2L3
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 169-327 aa (Q07820) were fused with C-terminal 6His tag.
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Sequence
MSKIKEEEDE LYRQSLEIIS RYLREQATGA KDTKPMGRSG ATSRKALETL RRVGDGVQRN HETAFQGMLR KLDIKNEDDV KSLSRVMIHV FSDGVTNWGR IVTLISFGAF VAKHLKTINQ ESCIEPLAES ITDVLVRTKR DWLVKQRGWD GFVEFFHVED LEGGLEHHHH HH
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 19.79 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 22 kDa band.
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Purity
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant MCL1 protein (His tag) SDS-PAGE
M: Marker; Lane 1: MCL1; Lane 2: BSA
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"