MARCKS-ED peptide

MARCKS-ED peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KKKKKRFSFKKSFKLSGFSFKKNKK-OH

H-Lys-Lys-Lys-Lys-Lys-Arg-Phe-Ser-Phe-Lys-Lys-Ser-Phe-Lys-Leu-Ser-Gly-Phe-Ser-Phe-Lys-Lys-Asn-Lys-Lys-OH

25

95.15%

C₁₄₇H₂₄₃N₄₁O₃₁

3080.75

Synthetic

Powder

MARCKS-ED is a 25-mer peptide derived from the effector domain of the intracellular membrane protein myristoylated alanine-rich C-kinase substrate. MARCKS-ED can recognize phosphatidylserine (PS) with a preference for highly curved vesicles in a sequence specific manner. In synthetic lipid vesicles, it preferentially binds to highly curved vesicles containing PS, as shown by cosedimentation assays and fluorescence enhancement and anisotropy assays. In complex biological systems, it can detect highly curved, PS-enriched extracellular vesicles in rat plasma and specifically bind to PS-exposing membranes in a C. elegans model, suggesting its ability to sense both shape and lipid composition of membranes. MARCKS-ED could become a prototype for a new generation of peptide sensors that can simultaneously detect both PS and curvature. It may facilitate investigations of critical biological events such as extracellular vesicles shedding and apoptosis, and potentially serve as a small peptide alternative to Annexin V in detecting PS-exposing membranes in live animals.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Morton LA, Yang H, Saludes JP, Fiorini Z, Beninson L, Chapman ER, Fleshner M, Xue D, Yin H. MARCKS-ED peptide as a curvature and lipid sensor. ACS Chem Biol. 2013 Jan 18;8(1):218-25. doi: 10.1021/cb300429e. Epub 2012 Oct 29. PMID: 23075500; PMCID: PMC3548944.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.