Recombinant MAPK5 protein (His tag)

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Recombinant Human MAPKAPK5 Fragment (219-473) is a high-purity, soluble protein designed for structural and biochemical studies. This fragment represents a stable, functionally relevant region of MAP kinase-activated protein kinase 5, a key serine/threonine kinase involved in tumor suppression, mTORC1 signaling, and post-transcriptional regulation. Ideal for kinase assays, interaction studies, and mechanistic research.

MAPKAPK5 is a tumor suppressor serine/threonine-protein kinase that plays critical roles in mTORC1 signaling, Ras-induced senescence, and post-transcriptional regulation of MYC. It phosphorylates multiple substrates including FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27, p53, and RHEB. Through FOXO3 phosphorylation, it promotes nuclear localization and expression of miR-34b/c, which regulate MYC translation. The kinase also functions as a negative regulator of mTORC1 by phosphorylating and inhibiting RHEB. MAPKAPK5 interacts with atypical MAPKs (ERK3/MAPK6 or ERK4/MAPK4) in a complex phosphorylation cascade and mediates HSP27 phosphorylation in response to PKA stimulation, leading to F-actin rearrangement. The protein localizes to both cytoplasm and nucleus, with phosphorylation-dependent translocation to the cytoplasm following activation through interaction with ERK3/MAPK6 or ERK4/MAPK4 or phosphorylation by PKA at Ser-115.