Recombinant M3K5 protein (His tag)

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Recombinant Human MAP3K5/ASK1 Protein Fragment (aa 1243-1374) is a high-purity, stable, and soluble protein fragment derived from Mitogen-activated protein kinase kinase kinase 5 (ASK-1), a key regulator of stress-induced signaling pathways. This fragment is ideal for structural studies, biochemical assays, and investigating MAP kinase cascade mechanisms.

The full-length MAP3K5/ASK1 protein is a serine/threonine kinase that plays a central role in the MAP kinase signal transduction pathway, mediating cellular responses to environmental stressors such as oxidative stress and inflammatory signals. It is essential for apoptosis, innate immunity, and cell fate determination, acting upstream of the JNK and p38 MAPK cascades to regulate transcription factors like AP-1. Located primarily in the cytoplasm and endoplasmic reticulum, MAP3K5/ASK1's interaction with 14-3-3 proteins confines it to perinuclear regions, influencing its activity. The provided fragment (aa 1243-1374) was selected for its stability and solubility, enabling focused research on this critical signaling component.