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Product Name
LYPLA1 antibody
- Documents
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Description
LYPLA1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in HeLa cells, human brain tissue, human liver tissue, mouse brain tissue, rat brain tissue, rat liver tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 25 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Acyl protein thioesterase 1 antibody; APT 1 antibody; APT1 antibody; hAPT1 antibody; LPL I antibody; LPL1 antibody; LYPLA1 antibody; Lysophospholipase 1 antibody; lysophospholipase I antibody; LYSOPLA antibody; LysoPLA I antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LYPLA1 recombinant protein (Accession Number: NM_006330). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112409(LYPLA1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112409(LYPLA1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112409(LYPLA1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-LYPLA1 (IP:Catalog No:112409, 4ug; Detection:Catalog No:112409 1:500) with mouse brain tissue lysate 4000ug.
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Background
LYPLA1(Lysophospholipase 1) is also named as APT1(Acyl-protein thioesterase 1), LPL1 and belongs to the AB hydrolase 2 family. It is an important enzyme responsible for depalmitoylation of palmitoyl proteins and works as a thioesterase that cleaves the S-palmitoyl group of H-Ras, heterotrimeric G protein alfa subunit (Ga) , regulator of G protein signaling 4 (RGS4) and the endothelial isoform of nitric oxide synthase (eNOS) in vitro(PMID:19439193).
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References
- Koyama K, Ogura Y, Nakai D. Identification of bioactivating enzymes involved in the hydrolysis of laninamivir octanoate, a long-acting neuraminidase inhibitor, in human pulmonary tissue. Drug metabolism and disposition: the biological fate of chemicals. 42(6):1031-8. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"