LYPLA1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in HeLa cells, human brain tissue, human liver tissue, mouse brain tissue, rat brain tissue, rat liver tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 25 kDa
ELISA, WB, IHC, IP
Human,Mouse,Rat; other species not tested.
Acyl protein thioesterase 1 antibody; APT 1 antibody; APT1 antibody; hAPT1 antibody; LPL I antibody; LPL1 antibody; LYPLA1 antibody; Lysophospholipase 1 antibody; lysophospholipase I antibody; LYSOPLA antibody; LysoPLA I antibody
This antibody was obtained by immunization of LYPLA1 recombinant protein (Accession Number: BC010397). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112409(LYPLA1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112409(LYPLA1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112409(LYPLA1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-LYPLA1 (IP:Catalog No:112409, 4ug; Detection:Catalog No:112409 1:500) with mouse brain tissue lysate 4000ug.
LYPLA1(Lysophospholipase 1) is also named as APT1(Acyl-protein thioesterase 1), LPL1 and belongs to the AB hydrolase 2 family. It is an important enzyme responsible for depalmitoylation of palmitoyl proteins and works as a thioesterase that cleaves the S-palmitoyl group of H-Ras, heterotrimeric G protein alfa subunit (Ga) , regulator of G protein signaling 4 (RGS4) and the endothelial isoform of nitric oxide synthase (eNOS) in vitro(PMID:19439193).
- Koyama K, Ogura Y, Nakai D. Identification of bioactivating enzymes involved in the hydrolysis of laninamivir octanoate, a long-acting neuraminidase inhibitor, in human pulmonary tissue. Drug metabolism and disposition: the biological fate of chemicals. 42(6):1031-8. 2014.
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