Lycosin-I peptide

Lycosin-I peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

RKGWFKAMKSIAKFIAKEKLKEHL

Arg-Lys-Gly-Trp-Phe-Lys-Ala-Met-Lys-Ser-Ile-Ala-Lys-Phe-Ile-Ala-Lys-Glu-Lys-Leu-Lys-Glu-His-Leu

24

97%

Synthetic

Powder

Active against Gram-Negative Bacteria E. coli CGMCC1.1369 (MIC 25 uM), S. dysemeriae CPCC160015, P. species CGMCC1.1651, P. aeruginosa CMCC10104, S. typhimurium CGMCC1.1174, E. aerogenes CGMCC1.2021, A. wieringae CGMCC1.2033, A. faecalis CGMCC1.924, K. pneumoniae CPCC160022, E. cloacae CGMCC1.58 (MIC 50 uM), P. rettgeri CMCC49256 with MIC in the range of 3.1-12.5 uM unless specified, Gram-Positive E. faecalis CGMCC1.100 (MIC 25 uM), B. subtilis CGMCC1.88, B. thuringiensis Israel H14 (MIC 12.5 uM), B. cereus CMCC1.126 (MIC 6.2 uM), S. aureus AB94004, C. glutamicum CGMCC1.818, S. epidermidis ACCC02744, M. luteus AB206228, T. halophilus CGMCC1.2492 (MIC 3.1 uM), and fungi Penicillium ACCC30106, A. flavus CGMCC3.6433 (MIC 3.1 uM), B. bassiana CGMCC3.3576 (MIC 6.2 uM), C. albicans CMCC98001, and S. cerevisiae CGMCC2.1619 (MIC 50 uM) (Tan et al. Current Molecular Medicine 2013, 13, 1-11). In addition, Lycosin-I displays anticancer activity both in vitro and in vivo. It adopts a helical conformation in TFE or SDS.The peptide appears to target bacterial membranes. It only showed 10% hemolysis at 100 uM. Provided by Liu Z. The source of Lycosin-I is Venom, Lycosa singorensis.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Liu Z, Deng M, Xiang J, et al. A novel spider peptide toxin suppresses tumor growth through dual signaling pathways. Curr Mol Med. 2012;12(10):1350-60.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.