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Product Name
Recombinant LSM5 protein (His tag)
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Description
Recombinant Human LSm5 (U6 snRNA-associated Sm-like protein LSm5) Fragment (aa 2-91) is a high-purity protein designed for studying pre-mRNA splicing mechanisms. This fragment fully encompasses the essential Sm domain, making it an ideal tool for investigating domain-specific functions in spliceosome assembly and U6 snRNA binding.
The full-length LSm5 protein, encoded by the LSM5 gene in Homo sapiens, plays a critical role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP complex, which is essential for spliceosome assembly. It also participates in the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex, including LSm5, specifically binds to the 3'-terminal U-tract of U6 snRNA, facilitating splicing regulation. LSm5 is localized exclusively in the nucleus, consistent with its nuclear functions in RNA processing.
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Protein name
U6 snRNA-associated Sm-like protein LSm5
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Uniprot ID
Q9Y4Y9
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Gene Name
LSM5
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 2-91 aa (Q9Y4Y9) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 11.46 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 12 kDa band.
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Purity
>87%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"