LPCL peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

• LPCL is a polypeptide with significant potential in the field of health and medicine. It has been identified as a promising inhibitor of dipeptidyl peptidase IV (DPP-IV). This polypeptide exhibits favorable pharmacokinetic properties, suggesting its potential for effective utilization within the body.
• In vitro studies have demonstrated that LPCL shows potent inhibitory activity against DPP-IV. It has an IC50 value of 76.56 ± 3.17μM, and it functions as a competitive inhibitor. Through various interactions such as electrostatic, π-stacking, hydrogen, and hydrophobic interactions with the DPP-IV catalytic triad, LPCL can effectively bind to the enzyme. Additionally, in situ experiments have shown that LPCL can significantly reduce the DPP-IV activity in STC-1 cells to 27.40 ± 2.88%, with a relatively low IC50 value of 481.6μM. Importantly, LPCL treatment has been shown to increase the levels of active GLP-1, which is crucial for glucose homeostasis. Overall, LPCL holds great promise as a potential agent for the management of conditions related to DPP-IV activity, such as type-2 diabetes mellitus.

• Rhessa Grace Guanga Ortizo, Vishal Sharma, Mei-Ling Tsai, Parushi Nargotra, Pei-Pei Sun, Chiu Wen Chen, Cheng-Di Dong, Integrating in silico, in vitro, and in situ approaches for characterization of novel peptides from tilapia (Oreochromis niloticus) viscera hydrolysates with dipeptidyl-peptidase IV inhibitory activity, Food Bioscience, Volume 63, 2025, 105619, ISSN 2212-4292, doi: org/10.1016/j.fbio.2024.105619.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.