LMWP - Low molecular weight protamine peptide

LMWP - Low molecular weight protamine peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

VSRRRRRRGGRRRR

Val-Ser-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Gly-Arg-Arg-Arg-Arg

14

96.21%

C₇₂H₁₄₂N₄₄O₁₆

1880.17

121052-30-0

Synthetic

Powder

Low molecular weight protamine (LMWP) is a peptide fragment derived from native protamine through enzymatic digestion with thermolysin. Its amino acid sequence is VSRRRRRRGGRRRR. It has several important properties and functions. LMWP can penetrate cell membranes and accumulate in the cytoplasm and nucleus. It has a similar cell transduction activity to the well-studied TAT47–57 peptide. This ability allows it to act as a cell-penetrating peptide (CPP), also known as a protein transduction domain (PTD), which is useful for intracellular delivery of various molecules. LMWP has been thoroughly studied in animal models. It is neither antigenic nor mutagenic, and it elicits only minimal complement activation and no detectable hypotensive or toxic responses.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Choi YS, Lee JY, Suh JS, Kwon YM, Lee SJ, Chung JK, Lee DS, Yang VC, Chung CP, Park YJ. The systemic delivery of siRNAs by a cell penetrating peptide, low molecular weight protamine. Biomaterials. 2010 Feb;31(6):1429-43. doi: 10.1016/j.biomaterials.2009.11.001. Epub 2009 Dec 1. PMID: 19954842.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.