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Product Name
LMP2 antibody
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Description
LMP2 Rabbit Polyclonal antibody. Positive IP detected in Raji cells. Positive WB detected in Raji cells, HepG2 cells, rat spleen tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human lymphoma tissue, human tonsillitis tissue. Observed molecular weight by Western-blot: 21-23 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
20S proteasome antibody; beta1i antibody; LMP2 antibody; Low molecular mass protein 2 antibody; Macropain chain 7 antibody; Proteasome chain 7 antibody; Proteasome subunit beta 1i antibody; Proteasome subunit beta type 9 antibody; PSMB6i antibody; PSMB9 antibody; RING12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LMP2 recombinant protein (Accession Number: NM_002800). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:112286(PSMB9 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:112286(PSMB9 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:112286(PSMB9 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-PSMB9 (IP:Catalog No:112286, 4ug; Detection:Catalog No:112286 1:300) with Raji cells lysate 2800ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:112286(PSMB9 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PSMB9, also named as LMP2, PSMB6i and RING12, belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. High expression of PSMB9 associated with the poor prognosis of patients with NPC.
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References
- Qifeng S, Bo C, Xingtao J, Chuanliang P, Xiaogang Z. Methylation of the promoter of human leukocyte antigen class I in human esophageal squamous cell carcinoma and its histopathological characteristics. The Journal of thoracic and cardiovascular surgery. 141(3):808-14. 2011.
- Zhao X, Sun Q, Tian H, Cong B, Jiang X, Peng C. Loss of heterozygosity at 6p21 and HLA class I expression in esophageal squamous cell carcinomas in China. Asian Pacific journal of cancer prevention : APJCP. 12(10):2741-5. 2011.
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