Lf-CATH2 peptide

Lf-CATH2 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GKCNVLCQLKQKLRSIGSGSHIGSVVLPRG-OH

H-Gly-Lys-Cys-Asn-Val-Leu-Cys-Gln-Leu-Lys-Gln-Lys-Leu-Arg-Ser-Ile-Gly-Ser-Gly-Ser-His-Ile-Gly-Ser-Val-Val-Leu-Pro-Arg-Gly-OH

30

95.44%

C₁₃₄H₂₃₆N₄₄O₃₈S₂

3135.69

Synthetic

Powder

Lf-CATH2 is a mature antimicrobial peptide (AMP) found in the amphibian Limnonectes fragilis. It is one of the two cathelicidins identified in this species, the other being Lf-CATH1. Lf-CATH2 is composed of 30 amino acids and has a higher percentage of alpha-helical structure compared to Lf-CATH1. Both Lf-CATH1 and Lf-CATH2 exhibit potent antimicrobial activities against a broad spectrum of microorganisms, including drug-resistant strains, while showing minimal hemolysis and cytotoxicity, making thempromising cadidates for antimicrobial applications. The antimicrobial activity of Lf-CATH1 appears to be stronger than that of Lf-CATH2, suggesting that the optimal percentage of alpha-helical structure may vary for different peptides.
Details: The sequence of Lf-CATH2 is 56.3% similar to Cathelicidin-PY. Lf-CATH2 is active against Gram-positive S. aureus (ATCC2592, IS1303, IS 1349) (MIC 2.27-9.08 uM), S. epidermidis (MIC 4.5 uM), P. acnes ATCC11827 (MIC 18.2 uM), Gram-negative P. aeruginosa (IS 1411, IS 1412, IS 1413) (MIC 4.5-9.1 uM), E. coli (ATCC25922, IS 1334, IS 1335, IS 1342) (MIC 2.3-9.1 uM), and fungi C. albicans ATCC2002, C. glabrata IS0902) (MIC 1.1-4.5 uM). Lf-CATH2 was found in fragile large-headed frog, fragile wart frog, Limnonectes fragilis, Hainan, China, Asia.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Yu H, Cai S, Gao J, et al. Identification and polymorphism discovery of the cathelicidins, Lf-CATHs in ranid amphibian (Limnonectes fragilis). FEBS J. 2013;280(23):6022-32.
• Lu Y, Gao J, Qiao X, Wang Y, Yu H. [Construction of novel thioredoxin fusion protein expression system and the production of recombinant Lf-CATH2]. Sheng Wu Gong Cheng Xue Bao. 2015 Mar;31(3):403-10.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.