Rat LIFR/CD118 (His Tag) recombinant protein

Rat LIFR/CD118 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp130, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (cardiotrophin like cytokine). Evidence showed that in the retina, LIFR activating LIF, CT-1 and cardiotrophin like cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF05) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.

Leukemia inhibitory factor receptor

LIFR; AW061234; FLJ99923; FLJ98106; LIF-R; SWS; STWS; SJS2; A230075M04RIK; CD118; LIF

G3V7K2

Lifr; rCG_50984

Human Cells

A DNA sequence encoding the rat LIFR (G3V7K2) (Met1-Ser829) was expressed with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat LIFR comprises 797 amino acids and predicts a molecular mass of 90 kDa. The apparent molecular mass of the recombinant protein is approximately 116 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

1. Measured by its ability to bind mouse LIF-Fc (Cat:50755-M02H) in a functional ELISA.
2. Measured by its ability to inhibit the recombinant human LIF mediated inhibition in the M1 mouse myeloid leukemia cells. The ED₅₀ for this effect is typically 8-40 ng/mL in the presence of 2 ng/mL recombinant human LIF.