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Product Name
Recombinant LDH protein (His tag)
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Description
Recombinant L-lactate dehydrogenase (LDH) (EC 1.1.1.27) fragment from Caenorhabditis elegans, comprising residues 1-333. This stable and soluble protein unit is ideal for structural studies, biochemical assays, and functional characterization of LDH activity.
L-lactate dehydrogenase (LDH) is a cytoplasmic enzyme that catalyzes the interconversion of lactate and pyruvate with concomitant interconversion of NADH and NAD+. As a key metabolic enzyme, LDH plays a crucial role in anaerobic glycolysis and cellular energy production. The cytoplasmic localization indicates its involvement in central metabolic pathways within the cell. The full-length protein from C. elegans (UniProt Q27888) provides a valuable model for studying conserved metabolic mechanisms across species.
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Protein name
L-lactate dehydrogenase
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Uniprot ID
Q27888
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Gene Name
ldh-1
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-333 aa (Q27888) were fused with 6His tag.
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Protein Species
Caenorhabditis elegans
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Molecular weight
Predictes a molecular mass of 37.72 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 38 kDa band.
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Purity
>88%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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