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Product Name
Recombinant KSR1 protein (His tag)
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Description
Recombinant Human Kinase Suppressor of Ras 1 (KSR1) Fragment (aa 605-880) - a highly stable and soluble protein unit ideal for structural studies and biochemical characterization of this key MAPK signaling regulator.
Kinase Suppressor of Ras 1 (KSR1) is a critical component of multiprotein signaling complexes that promote phosphorylation of Raf family members and activation of downstream MAP kinases. This protein functions as a MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF, where it dimerizes with BRAF upon binding to MEK proteins to enhance BRAF-mediated phosphorylation. KSR1 promotes activation of MAPK1 and MAPK3 in response to both EGF and cAMP signaling. The protein exhibits dynamic subcellular localization, transitioning from cytoplasmic sequestration in unstimulated cells (bound to 14-3-3 proteins) to membrane translocation at the cell periphery following growth factor stimulation, with presence at the cell membrane, ruffle membrane, and endoplasmic reticulum membrane.
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Protein name
Kinase suppressor of Ras 1
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Uniprot ID
Q8IVT5
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Gene Name
KSR1; KSR
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 605-880 aa (Q8IVT5) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 33.28 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 34 kDa band.
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Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"