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Product Name
KLF4 antibody
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Description
KLF4 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, HT-1080 cells, mouse testis tissue, NCCIT cell, NIH/3T3 cells, RAW264.7 cells. Positive IF detected in human embronic stem cells. Positive IHC detected in human colon tissue, human spleen tissue, human tonsil tissue. Observed molecular weight by Western-blot: 50-60 kDa
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Tested applications
ELISA, IHC, IP, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EZF antibody; GKLF antibody; KLF4 antibody; Krueppel like factor 4 antibody; Kruppel like factor 4 (gut) antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KLF4 recombinant protein (Accession Number: BC030811). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112081(KLF4 antibody) at dilution of 1:1000
IP Result of anti-KLF4 (IP:Catalog No:112081, 3ug; Detection:Catalog No:112081 1:500) with HeLa cells lysate 4650ug.
Immunohistochemical of paraffin-embedded human colon using Catalog No:112081(KLF4 antibody) at dilution of 1:50 (under 10x lens)
Confocal immunofluorescent analysis of human embronic stem cells with Catalog No:112081 at dilution of 1:50. The PE shows staining with Catalog No:112081/PE. The DAPI shows nuclear staining by DAPI.
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Background
The mammalian Krüppel-like transcription factor 4 (Klf4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. It also plays a significant role in the process of tumorigenesis. Klf4 is one of the key transcription factors that have been used to reprogram mouse and human fibroblasts to a pluripotent state also called induced pluripotent stem (iPS) cells. Affinity purified rabbit anti- Klf4 is for the detection of Klf4 transgene expression in the process of reprogramming. There are two isoforms of KLF4[PMID:15561714], with MW range from 50 to 60kDa,and major band is 55kDa.
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References
- Li W, Liu M, Su Y, Zhou X, Liu Y, Zhang X. The Janus-faced roles of Krüppel-like factor 4 in oral squamous cell carcinoma cells. Oncotarget. 6(42):44480-44494. 2015.
- Yin F, Lan R, Zhang X. LSD1 regulates pluripotency of embryonic stem/carcinoma cells through histone deacetylase 1-mediated deacetylation of histone H4 at lysine 16. Molecular and cellular biology. 34(2):158-79. 2014.
- Zhang X, Lu F, Wang J. Pluripotent stem cell protein Sox2 confers sensitivity to LSD1 inhibition in cancer cells. Cell reports. 5(2):445-57. 2013.
- Shan T, Zhou C, Yang R. Lithium chloride promotes the odontoblast differentiation of hair follicle neural crest cells by activating Wnt/β-catenin signaling. Cell biology international. 39(1):35-43. 2015.
- Zheng Y, Zou F, Wang J. Photodynamic therapy-mediated cancer vaccination enhances stem-like phenotype and immune escape, which can be blocked by thrombospondin-1 signaling through CD47 receptor protein. The Journal of biological chemistry. 290(14):8975-86. 2015.
- Kissing S, Hermsen C, Repnik U. Vacuolar ATPase in phagosome-lysosome fusion. The Journal of biological chemistry. 290(22):14166-80. 2015.
- Stavri S, Simionescu M, Kardassis D, Gafencu AV. Krüppel-like factor 4 synergizes with CREB to increase the activity of apolipoprotein E gene promoter in macrophages. Biochemical and biophysical research communications. 468(1-2):66-72. 2015.
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