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Product Name
KCNN3 Polyclonal Antibody
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Description
Polyclonal antibody to KCNN3
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
KCNN3 antibody; KCa2.3 antibody; SK3 antibody; SKCA3 antibody; hSK3 antibody; potassium calcium-activated channel subfamily N member 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 237-426 of human KCNN3 (NP_740752.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IF 1:50 - 1:100 -
Validations
Western blot - KCNN3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using KCNN3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 60s.
Immunofluorescence - KCNN3 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using KCNN3 antibody . Blue: DAPI for nuclear staining.
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Background
Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"