Recombinant ITPK1 protein (His tag)

• DataSheet
• Material Safety Data Sheets (MSDS)

Recombinant Human Inositol-tetrakisphosphate 1-kinase (ITPK1) fragment (amino acids 1-327) is a high-purity protein designed for studying inositol phosphate metabolism and signaling. This fragment fully encompasses the ATP-grasp functional domain, making it an ideal tool for investigating kinase activity, substrate specificity, and regulatory mechanisms in cellular processes including calcium signaling and programmed cell death pathways.

Inositol-tetrakisphosphate 1-kinase (ITPK1) is a multifunctional enzyme that phosphorylates various inositol polyphosphates including Ins(3,4,5,6)P4 and Ins(1,3,4)P3, playing a crucial role in the hexakisphosphate (InsP6) biosynthesis pathway. The enzyme demonstrates dual functionality as both a kinase and phosphatase, regulating the interconversion of inositol phosphate isomers. ITPK1 serves as the rate-limiting enzyme in the InsP6 pathway and modulates key cellular processes including TNF-alpha-induced apoptosis and MLKL-mediated necroptosis. By producing highly phosphorylated inositol phosphates such as InsP6, ITPK1 facilitates MLKL activation, oligomerization, and membrane localization during necroptosis, highlighting its significance in programmed cell death mechanisms and cellular signaling networks.