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Product Name
Recombinant ITPA protein (His tag)
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Description
Recombinant Inosine triphosphate pyrophosphatase (ITPase) from Caenorhabditis elegans, featuring a stable 1-184 amino acid fragment optimized for structural and biochemical studies. This enzyme efficiently hydrolyzes non-canonical purine nucleotides, serving as a critical tool for investigating nucleotide pool regulation and genome integrity mechanisms.
Inosine triphosphate pyrophosphatase (ITPase) functions as a pyrophosphatase that specifically hydrolyzes non-canonical purine nucleotides including inosine triphosphate (ITP), deoxyinosine triphosphate (dITP), and xanthosine triphosphate (XTP), converting them to their respective monophosphate derivatives. The enzyme exhibits no discrimination between deoxyribose and ribose forms of these nucleotides. ITPase plays a vital role in cellular nucleotide quality control by excluding non-canonical purines from RNA and DNA precursor pools, thereby preventing their incorporation into nucleic acids and avoiding potential chromosomal damage. The protein is localized to the cytoplasm where it maintains nucleotide pool integrity.
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Protein name
Inosine triphosphate pyrophosphatase
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Uniprot ID
Q9GYG4
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Gene Name
hap-1
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-184 aa (Q9GYG4) were fused with 6His tag.
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Protein Species
Caenorhabditis elegans
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Molecular weight
Predictes a molecular mass of 22.24 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 23 kDa band.
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Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"