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  • IRF9 antibody
  • IRF9 antibody
  • IRF9 antibody
  • IRF9 antibody

IRF9 antibody

Cat.#: 111842

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Special Price 239.0 USD

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Product Information

  • Product Name
    IRF9 antibody
  • Documents
  • Description
    IRF9 Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue, rat heart tissue. Positive IP detected in mouse heart tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human cervical cancer tissue. Observed molecular weight by Western-blot: 48kd
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    interferon regulatory factor 9 antibody; IRF 9 antibody; IRF9 antibody; ISGF 3 gamma antibody; ISGF3 antibody; ISGF3 p48 subunit antibody; ISGF3G antibody; p48 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of IRF9 recombinant protein (Accession Number: BC035716). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:111842(IRF9 antibody) at dilution of 1:500

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:111842(IRF9 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:111842(IRF9 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:111842(IRF9 antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using IRF9 antibody Catalog No:111842 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of HepG2 cells, using IRF9 antibody Catalog No:111842 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    IP Result of anti-IRF9 (IP:Catalog No:111842, 4ug; Detection:Catalog No:111842 1:500) with mouse heart tissue lysate 3200ug.

    IP Result of anti-IRF9 (IP:Catalog No:111842, 4ug; Detection:Catalog No:111842 1:500) with mouse heart tissue lysate 3200ug.

  • Background
    Interferon regulatory factor 9 (IRF9) also named Interferon-stimulated gene factor 3 gamma (ISGF3) is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
  • References
    • Zemp FJ, McKenzie BA, Lun X. Resistance to oncolytic myxoma virus therapy in nf1(-/-)/trp53(-/-) syngeneic mouse glioma models is independent of anti-viral type-I interferon. PloS one. 8(6):e65801. 2013.
    • Sun X, Wu Y, Gu M, Zhang Y. miR-342-5p decreases ankyrin G levels in Alzheimer's disease transgenic mouse models. Cell reports. 6(2):264-70. 2014.
    • Kroczynska B, Rafidi RL, Majchrzak-Kita B. Interferon γ (IFNγ) Signaling via Mechanistic Target of Rapamycin Complex 2 (mTORC2) and Regulatory Effects in the Generation of Type II Interferon Biological Responses. The Journal of biological chemistry. 291(5):2389-96. 2016.
  • cDNA Clones

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"