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Product Name
INSIG2 antibody
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Description
INSIG2 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in rat liver tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
INSIG 2 antibody; INSIG2 antibody; insulin induced gene 2 antibody; Insulin induced gene 2 protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of INSIG2 recombinant protein (Accession Number: XM_047444640). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
rat liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:111849(INSIG2 Antibody) at dilution of 1:1000
IP Result of anti-INSIG2 (IP:Catalog No:111849, 4ug; Detection:Catalog No:111849 1:500) with mouse liver tissue lysate 3600ug.
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:111849(INSIG2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:111849(INSIG2 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:111849(INSIG2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
INSIG2, also named as insulin induced gene 2, mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. INSIG2 may bring HMGCR into ubiquitin-mediated proteasomal degradation. Insig2 protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. INSIG-2 may play an important role in therapy of hypercholesterolemia.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"