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Product Name
IL5RA antibody
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Description
IL5RA Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas cancer tissue. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 43kd and 75kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CD125 antibody; CDw125 antibody; HSIL5R3 antibody; IL 5 receptor subunit alpha antibody; IL 5R alpha antibody; IL 5R subunit alpha antibody; IL 5RA antibody; IL5R antibody; IL5RA antibody; interleukin 5 receptor antibody; alpha antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IL5RA recombinant protein (Accession Number: NM_175728). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111746(IL5RA antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:111746(IL5RA antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using IL5RA antibody Catalog No:111746 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
IL5RA represents interleukin 5 receptor, alpha
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"