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IL1 beta antibody

Cat.#: 111653

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Special Price 239.0 USD

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Product Information

  • Product Name
    IL1 beta antibody
  • Documents
  • Description
    IL1 beta Rabbit Polyclonal antibody. Positive WB detected in A431 cells, human adrenal gland tissue, RAW 264.7 cells, Transfected HEK-293 cells. Positive IF detected in HeLa cells. Positive IHC detected in human skin tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue, human testis tissue, mouse heart tissue. Observed molecular weight by Western-blot: 30-35 kDa
  • Tested applications
    ELISA, IHC, IF, WB
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    IL1 beta antibody; Catabolin antibody; IL 1 antibody; IL 1 beta antibody; IL1 BETA antibody; IL1B antibody; IL1beta antibody; IL1F2 antibody; IL-1β antibody; Interleukin 1 beta antibody; interleukin 1 antibody; beta antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of IL1 beta recombinant protein (Accession Number: NM_000576). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111653(IL1B Antibody) at dilution of 1:700

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111653(IL1B Antibody) at dilution of 1:700

    Immunohistochemical of paraffin-embedded human skin using Catalog No:111653(IL1B antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human skin using Catalog No:111653(IL1B antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human skin using Catalog No:111653(IL1B antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human skin using Catalog No:111653(IL1B antibody) at dilution of 1:50 (under 40x lens)

    A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:111653(IL1B Antibody) at dilution of 1:300

    A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:111653(IL1B Antibody) at dilution of 1:300

    Immunofluorescent analysis of HeLa cells using Catalog No:111653(IL1B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HeLa cells using Catalog No:111653(IL1B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.
  • References
    • Xue H, Zhang XY, Liu JM, Song Y, Liu TT, Chen D. NDGA reduces secondary damage after spinal cord injury in rats via anti-inflammatory effects. Brain research. 1516:83-92. 2013.
    • Wang CP, Zhang LZ, Li GC. Mulberroside A protects against ischemic impairment in primary culture of rat cortical neurons after oxygen-glucose deprivation followed by reperfusion. Journal of neuroscience research. 92(7):944-54. 2014.
    • Zhang RN, Zheng B, Li LM, Zhang J, Zhang XH, Wen JK. Tongxinluo inhibits vascular inflammation and neointimal hyperplasia through blockade of the positive feedback loop between miR-155 and TNF-α. American journal of physiology. Heart and circulatory physiology. 307(4):H552-62. 2014.
    • Yan Y, Jiang W, Spinetti T. Omega-3 fatty acids prevent inflammation and metabolic disorder through inhibition of NLRP3 inflammasome activation. Immunity. 38(6):1154-63. 2013.
    • Xu D, Hu L, Su C. Tetrachloro-p-benzoquinone induces hepatic oxidative damage and inflammatory response, but not apoptosis in mouse: the prevention of curcumin. Toxicology and applied pharmacology. 280(2):305-13. 2014.
    • Wu X, He L, Chen F. Impaired autophagy contributes to adverse cardiac remodeling in acute myocardial infarction. PloS one. 9(11):e112891. 2014.
    • Owusu L, Wang B, Du Y, Li W, Xin Y. The quantum of initial transformed cells potentially modulates the type of local inflammation mechanism elicited by surrounding normal epithelial tissues and systemic immune pattern for tumor arrest or progression. Journal of Cancer. 6(2):128-38. 2015.
    • Peng K, Liu L, Wei D. P2X7R is involved in the progression of atherosclerosis by promoting NLRP3 inflammasome activation. International journal of molecular medicine. 35(5):1179-88. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"