Product Name

IL-17 antibody

Documents

Description

IL-17 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue, human prostate cancer tissue. Positive WB detected in U-937 cells, human testis tissue, mouse thymus tissue, mouse uterus tissue, Transfected HEK-293 cells. Positive IP detected in U-937 cells. Observed molecular weight by Western-blot: 32-35 kDa

Tested applications

ELISA, IP, WB, IHC

Species reactivity

Human, Mouse; other species not tested.

Alternative names

CTLA 8 antibody; CTLA8 antibody; IL 17 antibody; IL 17A antibody; IL17 antibody; IL-17 antibody; IL17A antibody; interleukin 17A antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of IL-17 recombinant protein (Accession Number: NM_002190). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IP: 1:200-1:1000

IHC: 1:50-1:500

Validations

U-937 cells were subjected to SDS PAGE followed by western blot with Catalog No:111762(IL17A antibody) at dilution of 1:500

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:111762(IL17A Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:111762(IL17A Antibody) at dilution of 1:200 (under 40x lens).

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111762(IL17A Antibody) at dilution of 1:500

IP Result of anti-IL17A (IP:Catalog No:111762, 3ug; Detection:Catalog No:111762 1:300) with U-937 cells lysate 6500ug.

Background

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased. This antibody got 32-35 kDa band in western blot, maybe due to homodimer formation and differential glycosylations.

References

Xie S, Ding L, Xiong Z, Zhu S. Implications of Th1 and Th17 cells in pathogenesis of oral lichen planus. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 32(3):451-7. 2012.
Rodero MP, Hodgson SS, Hollier B, Combadiere C, Khosrotehrani K. Reduced Il17a expression distinguishes a Ly6c(lo)MHCII(hi) macrophage population promoting wound healing. The Journal of investigative dermatology. 133(3):783-92. 2013.
Li Q, Li Q, Chen J. Prevalence of Th17 and Treg cells in gastric cancer patients and its correlation with clinical parameters. Oncology reports. 30(3):1215-22. 2013.
Ardeljan D, Wang Y, Park S. Interleukin-17 retinotoxicity is prevented by gene transfer of a soluble interleukin-17 receptor acting as a cytokine blocker: implications for age-related macular degeneration. PloS one. 9(4):e95900. 2014.
Gong Y, Li H, Li Y. Effects of Bacillus subtilis on Epithelial Tight Junctions of Mice with Inflammatory Bowel Disease. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research. 36(2):75-85. 2016.

IL-17 antibody

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