IGRP(206–214) peptide

IGRP(206–214) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-VYLKTNVFL-OH

H-Val-Tyr-Leu-Lys-Thr-Asn-Val-Phe-Leu-OH

9

95.46%

C₅₄H₈₅N₁₁O₁₃

1096.31

Synthetic

Powder

Peptide IGRP206-214 is a specific peptide derived from the IGRP (islet-specific glucose-6-phosphatase catalytic subunit-related protein) molecule. It is a 9-amino acid peptide with the sequence VYLKTNVFL. This peptide has been studied in the context of a single-chain peptide-MHC molecule called scKd.IGRP, which contains the heavy chain of H-2Kd, b2m, and the IGRP206-214 peptide. The scKd.IGRP complex adopts a conformation in which the covalently linked IGRP206-214 peptide is properly presented for specific recognition by CD8+ T cells bearing a cognate TCR. The binding capacity of scKd.IGRP to the cognate 8.3 TCR has been demonstrated, with scKd.IGRP tetramers binding to nearly all CD8+ T cells from 8.3 TCR-transgenic NOD mice. This indicates that the scKd.IGRP complex effectively presents the IGRP206-214 peptide for recognition by the cognate TCR.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Samanta D, Mukherjee G, Ramagopal UA, Chaparro RJ, Nathenson SG, DiLorenzo TP, Almo SC. Structural and functional characterization of a single-chain peptide-MHC molecule that modulates both naive and activated CD8+ T cells. Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13682-7. doi: 10.1073/pnas.1110971108. Epub 2011 Aug 8. PMID: 21825122; PMCID: PMC3158197.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.