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Product Name
IFITM1-Specific antibody
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Description
IFITM1-Specific Mouse Monoclonal antibody. Positive IHC detected in human lymphoma tissue. Positive WB detected in K562 cells, HeLa cells, K-562 cells. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 14kd; 17 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
9 27 antibody; CD225 antibody; IFI17 antibody; IFITM1 antibody; Interferon induced protein 17 antibody; Leu 13 antigen antibody; LEU13 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of IFITM1-Specific recombinant protein (Accession Number: NM_003641). Purification method: Caprylic acid/ammonium sulfate precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107275(IFITM1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:107275(IFITM1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:107275(IFITM1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-IFITM1-Specific (IP:Catalog No:107275, 4ug; Detection:Catalog No:107275 1:500) with HepG2 cells lysate 3600ug.
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Background
IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.
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References
- Zhang W, Zhang L, Zan Y, Du N, Yang Y, Tien P. Human respiratory syncytial virus infection is inhibited by IFN-induced transmembrane proteins. The Journal of general virology. 96(Pt 1):170-82. 2015.
- Tartour K, Appourchaux R, Gaillard J. IFITM proteins are incorporated onto HIV-1 virion particles and negatively imprint their infectivity. Retrovirology. 11:103. 2014.
- Xie M, Xuan B, Shan J. Human cytomegalovirus exploits interferon-induced transmembrane proteins to facilitate morphogenesis of the virion assembly compartment. Journal of virology. 89(6):3049-61. 2015.
- Compton AA, Bruel T, Porrot F. IFITM proteins incorporated into HIV-1 virions impair viral fusion and spread. Cell host & microbe. 16(6):736-47. 2014.
- Brass AL, Huang IC, Benita Y. The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus. Cell. 139(7):1243-54. 2009.
- Lin TY, Chin CR, Everitt AR. Amphotericin B increases influenza A virus infection by preventing IFITM3-mediated restriction. Cell reports. 5(4):895-908. 2013.
- Gao F, Liang H, Lu H. Global analysis of DNA methylation in hepatocellular carcinoma by a liquid hybridization capture-based bisulfite sequencing approach. Clinical epigenetics. 7(1):86. 2015.
- Lau DT, Negash A, Chen J. Innate immune tolerance and the role of kupffer cells in differential responses to interferon therapy among patients with HCV genotype 1 infection. Gastroenterology. 144(2):402-413.e12. 2013.
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