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Product Name
IDH2 antibody
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Description
IDH2 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in HeLa cells, HEK-293 cells, Jurkat cells, mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue, rat liver tissue, SH-SY5Y cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 43-47 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ICD M antibody; IDH antibody; IDH2 antibody; IDHM antibody; IDP antibody; IDPM antibody; mNADP IDH antibody; NADP(+) specific ICDH antibody; Oxalosuccinate decarboxylase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IDH2 recombinant protein (Accession Number: NM_002168). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111604(IDH2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111604(IDH2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111604(IDH2 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-IDH2 (IP:Catalog No:111604, 3ug; Detection:Catalog No:111604 1:1000) with mouse brain tissue lysate 6000ug.
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Background
IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis.
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References
- Agarwal AR, Zhao L, Sancheti H, Sundar IK, Rahman I, Cadenas E. Short-term cigarette smoke exposure induces reversible changes in energy metabolism and cellular redox status independent of inflammatory responses in mouse lungs. American journal of physiology. Lung cellular and molecular physiology. 303(10):L889-98. 2012.
- Wen H, Cho HR, Yun T. Metabolomic comparison between cells over-expressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism. Journal of neurochemistry. 132(2):183-93. 2015.
- Ma X, Li C, Sun L. Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. Nature communications. 5:5212. 2014.
- Chu B, Wu T, Miao L, Mei Y, Wu M. MiR-181a regulates lipid metabolism via IDH1. Scientific reports. 5:8801. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"