IARS Polyclonal Antibody
Polyclonal antibody to IARS
Human, Mouse, Rat
IARS antibody; GRIDHH antibody; IARS1 antibody; ILERS antibody; ILRS antibody; IRS antibody; PRO0785 antibody; isoleucyl-tRNA synthetase antibody
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1158-1262 of human IARS (NP_002152.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:50 - 1:100
Western blot - IARS Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IARS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
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