-
Product Name
IA-2/PTPRN antibody
- Documents
-
Description
IA-2/PTPRN Mouse Monoclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human brain tissue. Positive WB detected in Human brain, human brain tissue. Observed molecular weight by Western-blot: 60 kDa
-
Tested applications
ELISA, WB, IF, IHC
-
Species reactivity
Human; other species not tested.
-
Alternative names
FLJ16131 antibody; IA 2/PTP antibody; IA2 antibody; IA-2 antibody; ICA 512 antibody; ICA3 antibody; ICA512 antibody; Islet cell antigen 512 antibody; Islet cell autoantigen 3 antibody; PTP IA 2 antibody; PTPRN antibody; R PTP N antibody
-
Isotype
IgG3
-
Preparation
This antibody was obtained by immunization of IA-2/PTPRN recombinant protein (Accession Number: BC007713). Purification method: Protein A purified.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107271(PTPRN antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:107271(PTPRN Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:107271(PTPRN Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:107271(PTPRN Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG
-
Background
IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"