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Product Name
Hydroxyl-Histone H2A-Y39 Monoclonal Antibody
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Description
Monoclonal antibody to Hydroxyl-Histone H2A-Y39
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
HIST1H2AE antibody; H2A.1 antibody; H2A.2 antibody; H2A/a antibody; H2AFA antibody; histone H2A type 1-B/E antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A specific peptide of human Hydroxyl-Histone H2A-Y39
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:500 - 1:1000 -
Validations
Western blot - Hydroxyl-Histone H2A-Y39 Monoclonal Antibody
Western blot analysis of extracts of various cell lines, using Hydroxyl-Histone H2A-Y39 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - Hydroxyl-Histone H2A-Y39 Monoclonal Antibody
Immunohistochemistry of paraffin-embedded mouse colon using Hydroxyl-Histone H2A-Y39 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Hydroxyl-Histone H2A-Y39 Monoclonal Antibody
Immunohistochemistry of paraffin-embedded human tonsil using Hydroxyl-Histone H2A-Y39 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Hydroxyl-Histone H2A-Y39 Monoclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using Hydroxyl-Histone H2A-Y39 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Hydroxyl-Histone H2A-Y39 Monoclonal Antibody
Immunohistochemistry of paraffin-embedded mouse testis using Hydroxyl-Histone H2A-Y39 antibody at dilution of 1:100 (40x lens).
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Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"