Human tPA (Fc Tag) recombinant protein

Cat.#: 504208

Size:

Special Price 317.7 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Human tPA (Fc Tag) recombinant protein
  • Documents
  • Description
    Tissue plasminogen activator (abbreviated tPA or PLAT), is traditionally viewed as a simple serine protease whose main function is to convert plasminogen into biologically active plasmin. As a protease, tPA plays a crucial role in regulating blood fibrinolysis, in maintaining the homeostasis of extracellular matrix and in modulating the post-translational activation of growth factors. tPA is synthesized and secreted as a single chain polypeptide precursor which is cleaved in turn by plasmin. Proteolytic cleavage at the C-terminal side of Arg275 generates the enzyme composed of two subunits, designated as α and β chains which are held together by a single disulfide bond. Unlike the other members of the chymotrypsin family, tPA has one particular distinction in that the catalytic efficiency of the single-chain enzyme is only slightly lower than that of the proteolytically cleaved form and is therefore not a true zymogen. tPA is found not only in the blood, where its primary function is as a thrombolytic enzyme, but also in the central nervous system (CNS). It participats in a number of physiological and pathological events in the CNS, as well as the role of neuroserpin as the natural regulator of tPA's activity in these processes. Increased or decreased activity of tPA leads to hyperfibrinolysis or hypofibrinolysis, respectively. In addition, as a cytokine, tPA plays a pivotal role in the pathogenesis of renal interstitial fibrosis through diverse mechanisms. Thus, as a fibrogenic cytokine, it promotes the progression of kidney diseases.
  • Protein name
    Tissue-type plasminogen activator
  • Protein short names
    AW212668; AU020998; MGC18508; D8ERTD2E; PLAT; TPA; DKFZP686I03148; T-PA
  • Uniprot ID
    P00750
  • Gene Name
    PLAT
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the ? chain (Tyr 37-Arg 310) of mature human tPA (NP_000921.1) was expressed with the fused Fc region of human IgG1 at the N-terminus.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human Fc/tPA? is a disulfide-linked homodimeric protein. The reduced monomer consists of 512 amino acids and has a predicted molecular mass of 57.5 KDa. As a result of glycosylation, the apparent molecular mass of rh Fc/tPA? monomer is approximately 59 KDa in SDS-PAGE under reducing conditions.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Validations

    Human tPA / PLAT Protein (Fc Tag) SDS-PAGE

    Human tPA / PLAT Protein (Fc Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"