Human PUM1 Recombinant protein (GST tag)

Cat.#: 511760

Size:

Special Price 294.7 USD

Availability: 2-3 weeks
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Human PUM1 Recombinant protein (GST tag)
  • Documents
  • Description
    Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (PubMed:21572425, PubMed:18328718, PubMed:21653694, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:20818387, PubMed:20860814, PubMed:22345517). Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle (PubMed:20818387). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery (PubMed:25768905). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation (By similarity). {UniProtKB:Q80U78, PubMed:18328718, PubMed:18776931, PubMed:20818387, PubMed:20860814, PubMed:21397187, PubMed:21572425, PubMed:21653694, PubMed:22345517, PubMed:22955276, PubMed:25340845, PubMed:25768905}.
  • Protein name
    Pumilio homolog 1
  • Protein short names
    KIAA0099; PUMH1
  • Uniprot ID
    Q14671
  • Gene Name
    PUM1; KIAA0099; PUMH1
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human PUM1 (NP_001018494.1) 1-128 aa was fused with the N-terminal GST tag
  • Protein Species
    Human
  • Activity
    Not tested.
  • Validations

    Recombinant human PUM1 SDS-PAGE

    Recombinant human PUM1 SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"