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  • Human PMS2 Recombinant protein (His tag)

Human PMS2 Recombinant protein (His tag)

Cat.#: 511376

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Product Information

  • Product Name
    Human PMS2 Recombinant protein (His tag)
  • Documents
  • Description
    Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. {PubMed:16873062, PubMed:18206974, PubMed:23709753}.
  • Protein name
    Mismatch repair endonuclease PMS2
  • Protein short names
    PMSL2
  • Uniprot ID
    P54278
  • Gene Name
    PMS2; PMSL2
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    513-862 aa encoded by BC093921 was inserted into PET28a vector
  • Protein Species
    Human
  • Activity
    Not tested.
  • Validations

    Recombinant human PMS2 SDS-PAGE

    Recombinant human PMS2 SDS-PAGE

  • Antibodies

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"