Human Noggin (Fc Tag) recombinant protein

Human Noggin (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities, with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs, Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7, and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes, including embryonic dorsal-ventral axis, induction of neural tissue, formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development, joint formation, and neural tube fusion.

Noggin

SYNS1; SYM1; RP23-205A9.1; NOGGIN; NOG

Q13253

NOG

Human Cells

A DNA sequence encoding the human Noggin precursor (NP_005441.1) (Met 1-Cys 232) was fused with the Fc region of human IgG1 at the C-terminus.

Human

The recombinant human Noggin/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. Each monomer comprises 443 amino acids and has a predicted molecular mass of 49.8 kDa. As a result of glycosylation, the apparent molecular mass of rhNoggin/Fc monomer is approximately 58-62 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Measured by its ability to inhibit BMP-2 induced alkaline phosphatase production by MC3T3-E1 cells.
The ED₅₀ for this effect is typically 1.5-2.0 μg /mL in the presence of 0.25-0.5 μg/mL of BMP-2.
2. Measured by its ability to inhibit BMP4 induced alkaline phosphatase production by MC3T3-E1 cells.
The ED₅₀ for this effect is typically 0.1-0.6 μg/mL in the presence of 50 ng/mL of hBMP4.