Human IGFBP7 (Fc Tag) recombinant protein

Human IGFBP7 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Insulin like growth factor binding protein 7 (IGFBP7) is a member of the IGFBP family. It has been identified in colorectal adenocarcinoma (CRC) cell lines. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are clearly distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF, or act in an IGF-independent manner in the prostate. IGFBP7 could inhibit cell growth, decrease soft agar colony formation activity and induce apoptosis in RKO and SW620 cells. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.

Insulin-like growth factor-binding protein 7

RAMSVPS; PSF; MAC25; IGFBP7; IGFBP-7; AGM; TAF; IGFBP-7V; IBP-7; FSTL2; IGFBPRP1

Q16270

IGFBP7; MAC25; PSF

Human Cells

A DNA sequence encoding the human IGFBP7 (Q16270) (Met 1-Leu 282) was fused with the Fc region of human IgG1 at the C-terminus.

Human

The secreted recombinant human IGFBP7/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 497 amino acids and has a predicted molecular mass of 53.5 kDa. The apparent molecular mass of rh IGFBP7/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
1. Immobilized IGFBP7-Fc at 20 μg/ml (100 μl/well) can bind biotinylated human IGF2-nusa. The EC₅₀ of biotinylated human IGF2-nusa is 0.06 μg/ml.
2. Immobilized human CCL21 at 2 μg/ml (100 μl/well) can bind human IGFBP7-Fc with a linear ranger of 0.16-4 μg/ml.