Human Granzyme B/GZMB (His Tag) recombinant protein

Human Granzyme B/GZMB (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.

Q67BC3

Human Cells

A DNA sequence encoding the proform of human Granzyme B (NP_004122.1) (Met 1-Tyr 247) was expressed with a C-terminal polyhistidine tag.

Human

The recombinant human Granzyme B consists of 240 amino acids and has a calculated molecular mass of 27 kDa. As a result of glycosylation, the apparent molecular mass of rhGranzyme B is approximately 36 kDa in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE