Human G-CSFR/CD114 (His Tag) recombinant protein

Human G-CSFR/CD114 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.

Granulocyte colony-stimulating factor receptor

GCSFR; RP23-140F18.1; MGC141472; MGC141473; G-CSF-R; G-CSFR; CSFGR; CD114; CSF3R

Q99062

CSF3R; GCSFR

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Pro 621) of human G-CSF receptor (NP_000751.1) precursor was fused with a polyhistidine tag at the C-terminus.

Human

The mature recombinant human GCSFR consists of 608 amino acids and predicts a molecular mass of 68 kDa by SDS-PAGE under reducing conditions, the apparent molecular mass of rhGCSFR is approximately 92 kDa due to glycosylation.

> 85 % as determined by SDS-PAGE

Measured by its ability to inhibit the G-CSF induced proliferation of NFS60 mouse myeloid cells.
The ED₅₀ for this effect is typically 20-80 ng/mL.