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Product Name
Human Carbonic Anhydrase IV / Car4 / CA4 (His Tag) recombinant protein
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Description
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.
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Protein name
Carbonic anhydrase 4
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Protein short names
CARBONIC ANHYDRASE IV; CAIV; CA IV; CA4; CA-IV; RP17; CAR4; AW456718; RP23-167D6.1
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Uniprot ID
P22748
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Gene Name
CA4
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Source/Expression Host
CHO Stable Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human CA4 (NP_000708.1) (Met1-Lys283) without the pro peptide was expressed with a polyhistidine tag at the C-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human CA4 consists of 276 amino acids after removal of the signal peptide and has a predicted molecular mass of 31.7 kDa. In SDS-PAGE under reducing conditions, rh CA4 migrates as approximately 34 and 19 kDa band.
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Purity
(48.3+49.3) % as determined by SDS-PAGE
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Validations
Human Carbonic Anhydrase IV / CA4 Protein (His Tag) SDS-PAGE
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