Human 2B4 / CD244 (His Tag) recombinant protein

Human 2B4 / CD244 (His Tag) recombinant protein

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• Material Safety Data Sheets (MSDS)

The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.

Natural killer cell receptor 2B4

F730046O15RIK; NMRK; SLAMF4; C9.1; 2B4; CD244; NKR2B4; NMRK]; NAIL; LY90

Q9BZW8

CD244; 2B4

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Arg 221) of human 2B4 (NP_057466.1) was expressed, with a polyhistidine tag at the C-terminus.

Human

The recombinant 2B4 comprises 211 amino acids and predicts a molecular mass of 23.8 kDa. As a result of glycosylation, the rh 2B4 protein migrates as an approximately 45-50 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized human 2B4 at 2 μg/ml (100 μl/well) can bind human CD48. The EC₅₀ of human CD48 is 0.39 μg/ml.