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Product Name
Recombinant HTR5B protein (His tag)
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Description
Recombinant HEAT repeat-containing protein 5B (HEATR5B) fragment (residues 1579-2071) is a high-quality, stable protein designed for structural and functional studies. This 493-amino acid fragment, derived from human HEATR5B, is optimized for solubility and integrity, making it ideal for investigating clathrin-coated vesicle dynamics and protein trafficking mechanisms.
HEATR5B is a key component of clathrin-coated vesicles and the aftiphilin/p200/gamma-synergin complex, which regulates AP1G1/AP-1-mediated protein trafficking. It facilitates critical cellular processes, including transferrin transport from early to recycling endosomes and the trafficking of furin and cathepsin D between the trans-Golgi network and endosomes. The protein localizes to the perinuclear region and clathrin-coated vesicles, with its vesicular localization dependent on AFTPH/aftiphilin. This fragment represents a stable, soluble unit selected for structural and biochemical analyses, enabling detailed exploration of HEATR5B's role in intracellular transport pathways.
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Protein name
HEAT repeat-containing protein 5B
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Uniprot ID
Q9P2D3
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Gene Name
HEATR5B; KIAA1414; p200; p200a
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1579-2071 aa (Q9P2D3) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 55.37 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 55 kDa band.
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Purity
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"