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Product Name
HSD17B2 Polyclonal Antibody
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Description
Polyclonal antibody to HSD17B2
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Tested applications
WB
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Species reactivity
Mouse, Rat
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Alternative names
HSD17B2 antibody; EDH17B2 antibody; HSD17 antibody; SDR9C2 antibody; estradiol 17-beta-dehydrogenase 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 118-387 of human HSD17B2 (NP_002144.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - HSD17B2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HSD17B2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"