Recombinant HPSE protein (His tag)

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Recombinant Human Heparanase (EC 3.2.1.166) Fragment (residues 158-543) is a high-purity, soluble protein designed for structural and biochemical studies. This 386-amino acid fragment retains key functional regions, offering researchers a stable tool to investigate heparan sulfate cleavage, extracellular matrix remodeling, and roles in metastasis, inflammation, and angiogenesis.

Heparanase (HPSE) is an endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs), facilitating extracellular matrix degradation and remodeling under acidic conditions. It plays critical roles in tumor invasion, cell migration, wound healing, and inflammation by enhancing shedding of syndecans, promoting angiogenesis via VEGF activation, and acting as a procoagulant. Non-enzymatically, it supports cell adhesion and AKT1/PKB-mediated cell mobility. The full-length protein is synthesized as a proenzyme, secreted via Golgi vesicles, and processed in lysosomes into active 8 kDa and 50 kDa subunits, with localization in lysosomal membranes, endosomes, and occasionally the nucleus or cell surface. Its association with lipid rafts and HSPGs underscores its importance in pathological and physiological processes.