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Product Name
Human CD74 (His Tag) recombinant protein
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Description
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
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Protein name
HLA class II histocompatibility antigen gamma chain
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Protein short names
HLADG; IA-GAMMA; II; DHLAG; CD74; CLIP
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Uniprot ID
P04233
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Gene Name
CD74; DHLAG
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Gln 73-Met 232) of human CD74 isoform b (NP_004346.1) was fused with a polyhistidine tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human CD74 consists of 176 amino acids and has a calculated molecular mass of 20.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD74 is approximately 34 kDa due to glycosylation.
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Purity
> 80 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/ml (100 μl/well) can bind biotinylated human CTSL1 with a linear range of 3.2-400 ng/ml.
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Validations
Human CD74 Protein (His Tag) SDS-PAGE
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