HIP peptide
Not For Human Use, Lab Use Only.
Cat.#: 319283
Special Price 127.4 USD
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Product Name
HIP peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
H-GQVELGGGNAVEVLK-OH
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Sequence
H-Gly-Gln-Val-Glu-Leu-Gly-Gly-Gly-Asn-Ala-Val-Glu-Val-Leu-Lys-OH
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Length (aa)
15
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Peptide Purity (HPLC)
95.48%
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Molecular Formula
C63H108N18O22
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Molecular Weight
1469.63
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Source
Synthetic
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Form
Powder
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Description
HIP (hybrid insulin peptide) is a peptide derived from the splicing of insulin and islet amyloid polypeptides. It generates a preferred peptide-binding motif for HLA-DQ8, a genetic risk factor in type I diabetes. HIP is recognized by autoreactive CD4+ T cells, which are involved in the development of type I diabetes. Understanding the mechanism of recognition of HIP peptides by T cells can help in the development of therapies for type I diabetes. The crystal structure of TRBV5+ TCR-HLA-DQ8-HIP complexes provides insights into the recognition mechanism of HIP peptides by T cells, which can be useful in designing peptide-based therapies for type I diabetes.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
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References
- Tran MT, Faridi P, Lim JJ, Ting YT, Onwukwe G, Bhattacharjee P, Jones CM, Tresoldi E, Cameron FJ, La Gruta NL, Purcell AW, Mannering SI, Rossjohn J, Reid HH. T cell receptor recognition of hybrid insulin peptides bound to HLA-DQ8. Nat Commun. 2021 Aug 25;12(1):5110. doi: 10.1038/s41467-021-25404-x. PMID: 34433824; PMCID: PMC8387461.
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"