Recombinant GSKIP protein (His tag)

• DataSheet
• Material Safety Data Sheets (MSDS)

Recombinant GSK3B-interacting protein (GSKIP) fragment (amino acids 32-139) from Homo sapiens, designed for structural and biochemical studies. This 108-amino acid fragment represents a stable and soluble unit of GSKIP, an essential A-kinase anchoring protein that regulates GSK3B and PKA kinase activities in key cellular pathways including Wnt signaling and neuroprotection.

GSKIP (GSK3beta interaction protein) is a multifunctional A-kinase anchoring protein that forms ternary complexes with GSK3B and PKA to regulate their kinase activities toward specific targets. It plays crucial roles in Wnt-induced signaling by facilitating GSK3B- and PKA-mediated phosphorylation of beta-catenin, leading to its degradation and stabilization respectively. The protein contributes to neuroprotection against oxidative stress-induced apoptosis through PKA-mediated phosphorylation pathways and promotes neurite outgrowth and neuron proliferation. GSKIP localizes to both the cytoplasm and nucleus, where it modulates cell cycle progression by inhibiting GSK3B kinase activity and reducing N-cadherin levels. The selected fragment (32-139) represents a computationally optimized stable region suitable for investigating the structural basis of GSKIP's regulatory functions.