Recombinant GPT protein (His tag)

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Recombinant human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) fragment (residues 294-375), representing a stable and soluble unit of this essential enzyme involved in the initial step of protein N-glycosylation. This 82-amino acid fragment is ideal for structural studies and biochemical characterization of this key transferase in oligosaccharide biosynthesis.

The full-length DPAGT1 protein, also known as GlcNAc-1-P transferase or GPT, is a multi-pass membrane protein localized to the endoplasmic reticulum membrane. It catalyzes the critical initial step in the biosynthetic pathway of dolichol-linked oligosaccharides, which serve as glycan precursors for protein asparagine (N)-glycosylation. Specifically, this enzyme transfers GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate, producing GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane. This reaction represents the committed step in the assembly of dolichol-linked oligosaccharides, a process that begins on the cytosolic side of the endoplasmic reticulum and ultimately produces fourteen-sugar oligosaccharide precursors that are transferred to nascent proteins by oligosaccharyltransferases, making DPAGT1 fundamental to proper protein glycosylation and cellular function.