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Recombinant GPI8 protein (His tag)

Recombinant GPI8 protein (His tag)

Cat.#: 534855

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Product Information

  • Product Name
    Recombinant GPI8 protein (His tag)
  • Documents
  • Description

    This recombinant protein is a 331-amino acid fragment (residues 25-355) of the Putative GPI-anchor transamidase (PIG-K) from Drosophila melanogaster. It represents a stable, soluble unit ideal for investigating GPI anchoring mechanisms and conducting structural and biochemical studies.

    The full-length Putative GPI-anchor transamidase (PIG-K) plays a critical role in the endoplasmic reticulum by mediating GPI (glycosylphosphatidylinositol) anchoring. This enzyme facilitates the transamidation reaction that replaces a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI, forming a carbonyl intermediate with the substrate protein. This process is essential for attaching GPI anchors to proteins, enabling their membrane association and functional localization, as supported by similarity to characterized homologs (e.g., UniProtKB:Q92643).

  • Protein name
    Putative GPI-anchor transamidase
  • Uniprot ID
    Q8T4E1
  • Gene Name
    PIG-K
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    DNA encoding 25-355 aa (Q8T4E1) were fused with 6His tag.
  • Sequence 
    25-355
  • Protein Species
    Drosophila melanogaster
  • Molecular weight
    Predictes a molecular mass of 39.31 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 40 kDa band.
  • Purity
    >87%, by SDS-PAGE with Coomassie Brilliant Blue staining.
  • Activity
    Not tested.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"