GPAnt-2 peptide

GPAnt-2 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Pyr-QwFwwM-NH2, w=D-Trp

pGlu-Gln-D-Trp-Phe-D-Trp-D-Trp-Met-NH2

7

95.22%

C₅₇H₆₄N₁₂O₉S

1093.25

143675-79-0

71308571

Synthetic

Powder

GPAnt-2, has the amino acid sequence pGlu-Gln-D-Trp-Phe-D-Trp-D-Trp-Met-NH2, is a G-Protein antagonist peptide.
GPAnt-2 inhibited the activation of G proteins (Gi or Go) by M2 muscarinic cholinergic receptor (M2 mAChR) or of Gs by β-adrenergic receptor in the reconstituted phospholipid vesicles, as measured by receptor-promoted GTP hydrolysis.
The inhibition by GPAnt-2 was apparently reversible and competitive with respect to receptor binding to G proteins. The inhibition could be overcome by increasing the concentration of receptor in the vesicles and was not altered by changes in the concentration of G protein, suggesting that the peptide competes with the receptor for interaction with G proteins.
GPAnt-2 did not interfere with the binding of ligands to the receptor or the interaction of G protein and guanine nucleotide.
GPAnt-2 can be used as a tool to study the receptor-G protein interaction. For example, it was used to analyze the effect of an agonist on receptor-G protein coupling, and the results suggested that an agonist enhances the productive interaction between receptor and G protein by increasing the efficacy of coupling, not by increasing the affinity between receptor and G protein.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Mukai H, Munekata E, Higashijima T. G protein antagonists. A novel hydrophobic peptide competes with receptor for G protein binding. J Biol Chem. 1992 Aug 15;267(23):16237-43. PMID: 1379592.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.