Recombinant GNAI3 protein (His tag)

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This recombinant protein is a high-quality fragment of Human Guanine nucleotide-binding protein G(i) subunit alpha-3 (G(i) alpha-3), encompassing amino acids 2-354. It includes the complete G-alpha domains, making it an ideal reagent for investigating G protein-coupled receptor (GPCR) signaling pathways, adenylate cyclase inhibition, and ion channel regulation in cellular studies.

G(i) alpha-3, encoded by the GNAI3 gene, is a key heterotrimeric G protein alpha subunit that transduces signals from GPCRs. It functions by cycling between active GTP-bound and inactive GDP-bound states, modulating effectors such as adenylate cyclase—leading to reduced intracellular cAMP levels—and stimulating receptor-regulated K+ channels and TRPC5 calcium channels. The protein is primarily localized to the cell membrane via lipid anchoring and is also found in the cytoplasm, centrosomes, cleavage furrow, and midbody, implicating roles in cell division and spatial signaling regulation.