Recombinant GNAI1 protein (His tag)

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Recombinant Human GNAI1 (Guanine nucleotide-binding protein G(i) subunit alpha-1) fragment (residues 2-354) is a high-purity protein encompassing the complete G-alpha domains. This fragment serves as an essential tool for investigating G protein signaling, adenylate cyclase inhibition, and intracellular cAMP regulation, making it ideal for biochemical and functional studies.

Guanine nucleotide-binding protein G(i) subunit alpha-1, encoded by the GNAI1 gene, functions as a key transducer downstream of G protein-coupled receptors (GPCRs). It cycles between active GTP-bound and inactive GDP-bound states, modulating signaling cascades by inhibiting adenylate cyclases ADCY1, ADCY5, and ADCY6, thereby reducing intracellular cAMP levels. The protein also regulates cytokinesis, cortical dynein-dynactin complex recruitment during metaphase, and RGS14 translocation. It localizes to the nucleus, cytoplasm, cell membrane (as a peripheral membrane protein), centrosomes, cell cortex, and membranes via lipid anchors, with dynamic redistribution during mitosis and cytokinesis. The recombinant fragment (residues 2-354) fully contains the functional G-alpha domains, enabling targeted studies of its roles in GPCR signaling and cellular division.