Glucagon-Like Peptide I (7-36), amide, human

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Glucagon-Like Peptide I (7-36), amide, human, is a bioactive peptide hormone derived from the preproglucagon gene. It is secreted by intestinal L-cells in response to nutrient ingestion and plays a critical role in glucose homeostasis by enhancing insulin secretion and suppressing glucagon release. The peptide exerts its effects through binding to the GLP-1 receptor, a G-protein-coupled receptor widely expressed in pancreatic islets, the central nervous system, and other peripheral tissues.

The sequence of GLP-1 (7-36) amide consists of 30 amino acids, featuring key structural motifs essential for receptor binding and biological activity. It is rapidly degraded by the enzyme dipeptidyl peptidase-4 (DPP-4), resulting in a short plasma half-life. Despite its transient presence, GLP-1 (7-36) amide significantly influences energy metabolism, including the regulation of gastric emptying and satiety, through both peripheral and central mechanisms.

• G.I.Bell et al., Nature, 304, 368 (1983)
• B.Kreymann et al., Lancet, 2, 1300 (1987)
• C.Orskov and J.H.Nielsen, FEBS Lett., 229, 175 (1988)
• J.-P.Raufman et al., J. Biol. Chem., 267, 21432 (1992)
• P.A.Martin and A.Faulkner, Comp. Biochem. Biophys., 105A, 705 (1993)
• H.C.Fehmann et al., Peptides, 15, 453 (1994)
• M.A.Nauck et al., Exp. Clin. Endocrinol. Diabetes, 105, 187 (1997)

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.